Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles

In this study, C-doped TiO2 nanoparticles (C-TiO2) were prepared and tested as a photosensitizer for visible-light-driven photodynamic therapy against cervical cancer cells (HeLa). X-ray diffraction Transmission Electron Microscopy confirmed the anatase form of nanoparticles, spherical shape, size distribution from 5 to 15 nm. Ultraviolet–visible light spectroscopy showed that C doping enhances optical absorption in visible range caused by bandgap narrowing. The photo-cytotoxic activity C-TiO2 was investigated vitro HeLa cells. lack dark cytotoxicity indicates good biocompatibility C-TiO2. contrast, combination with blue significantly reduced survival cells: illumination only decreased cell viability 30% (15 min illumination, 120 µW power), 60% when preincubated We have also light-induced C-TiO2-catalyzed generation reactive oxygen species intracellularly. Oxidative stress triggered C-TiO2/blue leading cause death. Fluorescent labeling treated distinct morphological changes after treatment. Unlike which appearance large necrotic deformed nuclei, cytoplasm swelling, membrane blebbing, leads controlled death, thus providing better outcome local anticancer therapy.